Interleukin -10 – 1082A/G single nucleotide polymorphism and risk of Type 2 Diabetes Mellitus in a Southwestern population of Nigeria
Type 2 diabetes mellitus (T2DM) is a metabolic, multifactorial disease caused by genetic, dietary and environmental interactions with alterations in the pattern of cytokine expression. Association between interleukin (IL)-10 polymorphism and T2DM is not known in Black Africans. This study therefore investigates the relationship between IL-10 -1082A/G polymorphism and risk of T2DM in Southwest Nigeria. 100 patients with T2DM and 105 healthy controls were enrolled. Anthropometric indices were measured and fasting blood sample was obtained from all subjects. Plasma glucose and lipid profile were estimated by standard laboratory procedures. IL-10 (-1082A/G) gene polymorphism was genotyped using Allele Refractory Mutation detection System-Polymerase Chain Reaction (ARMS-PCR) technique. Odds ratio analysis (95%CI) was used to determine association between genotype and diabetes mellitus in studied subjects. T2DM patients had the highest frequency of IL 10 -1082G/G genotype (58.3%) while -1082AG frequency was highest (63.1%) in non-diabetics. Non- significant associations between IL 10 -1082 gene polymorphism and T2DM risk (for AG genotype: OR=0.95, 95%CI =0.19-4.2, p = 0.95; for GG genotype: OR=0.49, 95%CI = 0.10-2.41, p = 0.38) were recorded. Fasting plasma glucose, 2hours postprandial, total cholesterol and triglyceride were all significantly elevated in IL 10 -1082G/G than AG and AA genotypes in T2DM subjects (p<0.001). Expression of A allele was higher in controls than DM patients. This case-control study did not find a significant association between IL 10-1082A/G polymorphism and T2DM; however further studies with larger sample size and IL-10 plasma level measurement will be recommended to validate this observation.
- : Ayelagbe | G. | O | Department of Chemical Pathology, College of Health Sciences, Ladoke Akintola University of Technology, Osogbo, Nigeria | firstname.lastname@example.org | +234 806 561 5202, Daodu | O. | O | Department of Chemical Pathology, College of Health Sciences, Ladoke Akintola University of Technology, Osogbo, Nigeria | | , Akanbi | A. | O | Department of Medical Microbiology and Parasitology , College of Health Sciences, Ladoke Akintola University of Technology, Osogbo, Nigeria | | , Onuegbu | J. | A | Department of Chemical Pathology , Nnamdi Azikiwe University, Awka, Nigeria | | , Ojurongbe | | O | Department of Medical Microbiology and Parasitology , College of Health Sciences, Ladoke Akintola University of Technology, Osogbo, Nigeria | |